Assessment of zootechnical parameters, intestinal digestive enzymes, haemato-immune responses, and hepatic antioxidant status of Pangasianodon hypophthalmus fingerlings reared under different stocking densities

Abstract A 3-month experiment was designed to investigate the effects of different stocking densities on growth, stress markers, intestinal digestive enzymes, hepatic antioxidant biomarkers, serum immunity, and survivability striped catfish, Pangasianodon hypophthalmus . The relationship between fish water quality parameters also assessed. Healthy ( n = 1260 individuals, 17.52 ± 0.20 g) were raised in twelve fiberglass tanks. Triplicate groups with 60, 90, 120, or 150 per 1 m 3 designed, further referred as SD60, SD90, SD120, SD150 groups. Fish feeding done using a commercially purchased well-balanced diet three times day rate 3% wet weight. Results revealed that un-ionized ammonia, nitrite, pH significantly elevated, dissolved oxygen levels decreased increasing densities. survival rates growth lipase, protease, trypsin, amylase enzymes P < 0.05) along increased Moreover, significant decreases noticed total protein, lysozyme activity, globulin when density higher than 60 fish/m Conversely, biomarkers (such blood glucose cortisol), transaminases, alkaline phosphatase, urea nitrogen elevated Hepatic CAT, SOD, T-AOC decreased; meanwhile, MDA increased, together rates. In end, we found group recorded lowest rates, immune responses, capacity highest markers cortisol. this context, can conclude resulted better survival, status, overall performances catfish. Graphical